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IN VITRO
 HISTOLOGY ONCOLOGY SIDE EFFECTS
IN VITRO
RODENT PRIMARY CULTURE
ONCOLOGY
MODELS
ASSAYS
NEUROFIT offers a large range of models and assays for evaluating neurotoxicity, neuroprotection or neurotrophic activity of new test compounds.

NEUROFIT can also develop custom protocols to determine mechanism of action, or define therapeutic indication of new drug candidates.
RODENT PRIMARY CULTURE
ONCOLOGY
Cortical, Hippocampal, Mesencephalon neurons
Spinal cord motor neurons
Sensory neurons (dissociated DRG)
Schwann cells / sensory neurons co-culture
Rat nerve - human muscle co-culture
Cancer cell lines proliferation assay
MODELS
ASSAYS
Basal neuronal survival:
Cortical neurons
Spinal motor neurons
Exitotoxicity: intoxication with glutamate or NMDA
Cortical neurons
Spinal motor neurons
Apoptosis: staurosporine intoxication
Anti-mitotic intoxication:
Purified sensory neurons intoxicated with cisplatin, vincristine or taxol
Sensory neurons and schwann cells coculture intoxicated with cisplatin, vincristine or taxol
Spinal motor neurons intoxicated with vincristine
Alzheimer's models: intoxication of neuronal cultures with fibrillar amyloid beta peptide (Aβ)
Parkinson's models: mesencephalon neurons intoxicated with MPP+
Proliferation of Schwann cells
Neurotrophic effect: effects of test compounds on neurite outgrowth
Cortical neurons
Hippocampal neurons
Spinal motor neurons
Co-culture nerve/muscle: effects of test compounds on co-culture innervation
Apoptosis evaluation: measurement of caspase 3 activity
Co-culture: length of neuritis, surface of innervated muscle fiber area, video samples
Immunocytology: measurements of e.g. tyrosine hydroxylase, neurofilaments, Schwann cells (S-100 antibody), Hoechst nucleus
Neurotrophic effect: neurite length, percentage of cells displaying neuritis
Cortical neurons
Hippocampal neurons
Spinal motor neurons
Viability and survival tests: LDH, AP, MTT assays
AP assay
Release of LDH, MTT activity