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EAE is a commonly used animal model which shares some degree of similarities with human multiple sclerosis (MS).
EAE is induced by CNS antigens such myelin basic protein (MBP) which activates autoreactive T-cells responsible for CNS inflammation and demyelination. Within 10 days after the immunization with MBP of susceptible Lewis rats they develop a progressive weight loss and paralysis that can be assessed using semi-quantitative neurological scoring system (0 = no abnormality; 1 = distal weakness of the tail; 2 = complete weakness of the tail; 3 = mild weakness in one or two hindlimbs; 4 = moderate paraparesia of one or two hindlimbs; 5 = total paraplegia).
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Compound testing addresses the effect of subchronic or chronic treatment of rats (typically starting the before the appearance of first symptom) on:
- neurological score until complete recovery
- body weight
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