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Sciatic nerve crush is widely accepted as a valid model for peripheral nerve regeneration. In this model, indeed, nerve damage results in rapid disruption of nerve function as evidenced by electromyography measurement. Recovery of nerve function occurs within 2 weeks and by week 4 post-lesioning significant remyelination of the regenerated axons is observed in the sciatic nerve by histology. This model is responsive to various neuroprotective compounds [e.g., 4-methylcatechol (4-MC)], which accelerate both axon remyelination and functional recovery.
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Compound testing addresses the effect of chronic treatment of mice (typically starting the same as the nerve crush lesioning) on:
- recovery of nerve function (weekly electrophysiology measures) and
- axonal regeneration (morphometric analysis at week 3 or week 4 post-crush).
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Change in the amplitude of compound muscle action potential following sciatic nerve crush.
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Change in the latency of compound muscle action potential following sciatic nerve crush.
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Remyelination of regenerating nerve fibers 4 weeks after lesioning as indicated by g-ratio decrease.
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Change in the axon size 4 weeks following sciatic nerve crush.
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Fournier J., Steinberg R., Gauthier T., Keane P., Guzzi U., Coude F., Bougault I., Maffrand J.P., Soubrié P. and Lefur G. Protective effects of SR57746A in central and peripheral models of neurodegenerative disorders in rodents and primates. Neurosciences 55: 629-641. 1993.
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Lewis ME, Neff NT, Contreras PC, Stong DB, Oppenheim RW, Grebow PE, Vaught JL. Insulin-like growth factor-I: potential for treatment of motor neuronal disorders. Exp Neurol 124(1):73-88. 1993.
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Friedman B., Kleinfeld D., Verge V., Moulton R., Boland P., Zlotchenko E., Lindsay R. and Liu L. BDNF and NT4-5 exert neurotrophic influences on injured adult spinal motor neurons. J. Neurosciences. 15: 1044-1056. 1995.
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Strand FL, Kung TT. ACTH accelerates recovery of neuromuscular function following crushing of peripheral nerve. Peptides, 1(2):135-8. 1980.
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Laird JM, Mason GS, Thomas KA, Hargreaves RJ, Hill RG. Acidic fibroblast growth factor stimulates motor and sensory axon regeneration after sciatic nerve crush in the rat.
Neuroscience, 65(1):209-16. 1995
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