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DTH is immune reaction in which memory T-cells play a pivotal role. The reaction is antigen specific and causes erythema, oedema and induration at the site of antigen injection in immunized animals or humans.
DTH is induced in female Lewis rats by subcutaneous injection of emulsion of ovalbumin/complete Freund's adjuvant. One week later, rats are challenged with injection of ovalbumin in the pinna ear. The DTH reaction is determined by measuring the increase in the pinna ear thickness 24 hours after the ovalbumin challenge.
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