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Survival assay are widely used in neurobiology to study the neurotophic effect of a new compound.
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A primary culture of spinal motor neurons are plating at low density in a defined medium. The compound is added and after 72h the number of surviving neurons is evaluated by an enzymatic assay measuring acid phosphatase activity.
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Dose response curve from different neurotrophines on spinal motor neurons survival after 3 days of treatment. BDNF and bFGF are able to promote spinal motor neurons survival whereas GDNF and NGF are not.
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Ueda Y., Walsh E., Nakanishi H. and Yoshida K. A colorimetic assay method for evaluation of neurotrophic activity in vitro. Neurosci. Letters (1994) 165:203-207.
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Martin A. and Clynes M. Acid phosphatase: endpoint for in vitro toxicity tests. In Vitro Cell.Dev. Biol. (1991) 27A:183-184.
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