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EXCITOTOXICITY on SPINAL MOTOR NEURONS
Model of EXCITOTOXICITY on SPINAL MOTOR NEURONS
INTRODUCTION
The role of excitotoxicity in the neurological disorders is now recognised. Glutamate receptors activation contributes greatly in mediating injury to motor neurons. In vitro, a brief exposure to glutamate causes neuronal death mainly by excessive stimulation of NMDA receptors (Choi et al., 1988).
COMPOUND TESTING
Spinal neuron cultures are injured by an acute intoxication with glutamate or NMDA. The neuroprotective effect of compounds is evaluated based on their ability to inhibit the damage. In the pre-treatment protocol, test compound is added before intoxication and in the post-treatment one, test compound is added immediately after intoxication. Neuronal death is assessed by measuring LDH activity (Lactate deshydrogenase) in the media after glutamate/NMDA exposure and/or neuronal survival is assessed by measuring MTT activity after excitotoxic damage.
Release of LDH in response to increasing doses of acute glutamate intoxication on culture of spinal motor neuron
Release of LDH in response to increasing doses of acute NMDA intoxication on culture of spinal motor neuron
Neuronal survival (MTT activity) in response to increasing doses of acute glutamate intoxication on culture of spinal motor neuron
Neuronal survival (MTT activity) in response to increasing doses of acute NMDA intoxication on culture of spinal motor neuron
REFERENCES
CHOI D.W., KOH J. and PETERS S. (1988). Pharmacology of glutamate neurotoxicity in cortical cell cultures: attenuation by NMDA antagonists.
J. Neurosci., 8, 185-196.
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