EAE is a commonly used animal model which shares some degree of similarities with human multiple sclerosis (MS).
EAE is induced by CNS antigens such myelin basic protein (MBP) which activates autoreactive T-cells responsible for CNS inflammation and demyelination. Within 10 days after the immunization with MBP of susceptible Lewis rats they develop a progressive weight loss and paralysis that can be assessed using semi-quantitative neurological scoring system.

Compound testing addresses the effect of subchronic or chronic treatment of rats (typically starting before the appearance of first symptom) on:

- neurological score until complete recovery
- body weight