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SCIATIC NERVE CRUSH
SCIATIC NERVE CRUSH in mice
INTRODUCTION
Sciatic nerve crush is widely accepted as a valid model for peripheral nerve regeneration. In this model, indeed, nerve damage results in rapid disruption of nerve function as evidenced by electromyography measurement. Recovery of nerve function occurs within 2 weeks and by week 4 post-lesioning significant remyelination of the regenerated axons is observed in the sciatic nerve by histology.
COMPOUND TESTING
Compound testing addresses the effect of chronic treatment of mice (typically starting the same day as the nerve crush injury) on:

- recovery of nerve function (weekly electrophysiology measures) and
- axonal regeneration (morphometric analysis at week 3 or week 4 post-crush).

Graph showing the disruption of g-ratio distribution profile in sural nerve following a sciatic nerve crush. In sham operated specimen, g-ratio (relative thickness of the myelin sheath) is relatively constant for axons of all size. Note the presence of large number of small and hypermyelinated axons following sciatic nerve crush
(3 weeks post-injury).
Comparative photomicrograph of sural nerve sections showing the difference in the status of nerve fibers of Sham operated and sciatic nerve crush specimens at 3 weeks post-injury.

Note the marked absence of large caliber axons (arrows) in the nerve crushed specimen.

Graph showing the disruption of axon size distribution profile in sural nerve following a sciatic nerve crush (3 weeks post-injury).
Note the presence of polyphasia and temporal dispersion in the CMAP at Day 14 post-injury.
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