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BASAL CORTICAL NEURON SURVIVAL
Effect of compounds on BASAL CORTICAL NEURON SURVIVAL
INTRODUCTION
Survival assay are widely used in neurobiology to study the neurotophic effect of a new compound.
COMPOUND TESTING
A primary culture of cortical neurons are plated in a defined medium. The compound is added and after 24h, 48h and/or 72h the number of surviving neurons is evaluated by an enzymatic assay measuring acid phosphatase activity
Dose response curve from different neurotrophines on cortical neurons survival after 3 days of treatment.
BDNF and bFGF are able to promote cortical neurons survival whereas GDNF and NGF are not.
Effect of BDNF (50 ng/ml) or bFGF (5 ng/ml)
on cortical neurons survival after 24h, 48h or 72h of treatment.
REFERENCES
Ueda Y., Walsh E., Nakanishi H. and Yoshida K. A colorimetic assay method for evaluation of neurotrophic activity in vitro.
Neurosci. Letters (1994) 165:203-207.
Martin A. and Clynes M. Acid phosphatase: endpoint for in vitro toxicity tests.
In Vitro Cell.Dev. Biol. (1991) 27A:183-184.
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